Restriction Fragment Length Polymorphism (RFLP) typing of Mycobacterium tuberculosis isolates from Nepal

Abstract

Introduction: Many studies to characterize Mycobacterium tuberculosis by RFLP have shown that predominance of Beijing genotype in China and many neighboring countries. Nepal is just bordering in southern part of China. We have tried to differentiate Mycobacterium tuberculosis isolates of Nepal by RFLP in first time in Nepal.

Methods: A standard protocol was followed for isolation, Identification and RFLP typing of 60 strains of Mycobacterium tuberculosis of Nepal.

Results: Of the 60 isolates interpretable result was found in 31 isolates. The study detected that the Beijing family 12 (38.7%), was predominant type followed by single band 12 (25.5%). Indigenous type, not reported from other part of the world was also detected in 7 strains (22.6%).

Conclusion: Beijing type is the predominant RFLP type of Mycobacterium tuberculosis in Nepal and some other endogenous type also prevails which needs further study.